The laboratory of chemical and analytical methods is equipped in accordance with the requirements of GLP, equipped with necessary modern analytical and research instrumentation sufficient to perform titrimetric, gravimetric, spectroscopic and chromatographic analyses.
In particular the laboratory have:
two HPLC LC-20 Prominence systems (Shimadzu, Japan) equipped with diode array detectors, autosampler, chromatographic oven, besides one of the systems have a refractive index detector in addition. Both systems are computerized, data collecting and processing is carried out by LabSolutions software (Shimadzu, Japan).
recording spectrophotometer of UV and visible range, UV-1700 PharmaSpec (Shimadzu, Japan). The device is computerized, data collecting and processing is carried out by UV Probe software (Shimadzu, Japan).
system for HPTLC includes applicator for applying samples to the plate of TSH Gamag Linomat 5, Camag TLC scanner 3 and WinCats software (Camag, Switzerland).
system of water purification -Simplicity Water Purification System (Millipore,USA) to produce water of 1 type (qualification “for a chromatography”).
rotary evaporator IKA RV10 (IKA evaporator, Germany), ScanSpeed vacuum centrifuge (concentrator) (LaboGene, Denmark), various centrifuges and other equipment that is used in preparation of analytical tests.
Members of group conduct pharmacognostical and phytochemical studies of medicinal plants and extracts and perform:
pharmacognostical and merchandising analyses of medicinal plants that include microscopic examination, estimation of ash, humidity and impurity content;
phytochemical analysis of medicinal plants and products of its processing aimed at examing the main groups of active substances including phenolics and tannins, flavonoids and phenolic acids, coumarins, saponins, organic acids, amino acids, polysaccharides and sugars, fatty acids, ascorbic acid, carotenoids, tocopherols, phytosterols, and others;
comparative phytochemical analysis of raw materials received from wild or cultivated plants of various grades after additional processing at preparation and drying, etc.;
development/adoption of methods of analysis and standardization of medicinal plants and phytopreparations;
validation of analytical methods;
preparation of draft regulations (Manufacturer monograph, Technical specification) about raw materials, pharmaceuticals, biologically active additives (BAA), functional nutrition, etc. for pharmaceutical, food and cosmetic industry.
Preparation of draft regulations is carried out according to the following requirements:
State Pharmacopoeia of the Russian Federation. XIV ed., Moscow: Ministry of Health of the Russian Federation, 2018, Vol. 1-4. 7019 p.
Publications on topic:
Косман В.М., Зенкевич И.Г. Количественное экстракционно-спектрофотометрическое определение суммарного содержания гидроксикоричных кислот в присутствии флавоноидов в экстрактивных веществах некоторых растений // Раст. ресурсы, 2001, Т. 37, Вып. 4, С. 123-130.
Косман В.М., Пожарицкая О.Н., Шиков А.Н, Зенкевич И.Г., Пименов А.И., Макаров В.Г. Особенности химической характеристики экстрактивных водорастворимых компонентов семян Pinus sibirica Du Tour // Раст. ресурсы, 2001, Т. 37, Вып. 4, С. 81-89.
Зенкевич И.Г., Косман В.М., Макаров В.Г., Дадали Ю.B. Количественный рефракто-спектрофотометрический анализ масляных растворов витаминов «А» и «Е» // ЖПХ, 2001, Т. 74, Вып. 6, С. 1005-1010.
Шиков А.Н., Косман В.М., Пожарицкая О.Н. Сорбционные свойства шрота семян Pinus sibirica Du Tour // Растит. ресурсы, 2002, Т.38, вып.2, С.106-112.
Косман В.М., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Изучение экстракции иридоидных гликозидов травы пустырника различными растворителями // ХФЖ., 2002, Т. 36., № 2, С. 43-45.
Косман В.М., Пожарицкая О.Н., Станкевич Н.М., Шиков А.Н. Шрот корней женьшеня – перспективное сырье для получения энтеросорбента // Материалы Всероссийского семинара «Новые достижения в химии и химической технологии растительного сырья». Барнаул, 2002, С.130-133.
Pozharitskaya O.N., Ivanova S.A., Shikov A.N., Makarov V.G. Separation and quantification of terpenoids of Boswellia serrata extract by planar chromatography techniques (TLC and AMD) // J. Sep.Sci. 2006. Vol. 29. P. 2245-2250.
Косман В.М., Фаустова Н.М., Зенкевич И.Г., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Сравнительная характеристика состава образцов масла зародышей пшеницы различного происхождения // Масложировая пром-ть, 2007, №6. С. 32-34.
Косман В.М., Станкевич Н.М., Макаров В.Г., Тихонов В.П. Биологически активные вещества какао тертого и масла какао // Вопросы питания, 2007, № 3, С. 62-67.
Pozharitskaya O.N., Kosman V.M., Shikov A.N., Demchenko D. E., Eshchenko A.Yu. Makarov V.G.. Comparison between HPLC and HPTLC-densitometry for the determination of icariin from Epimedium koreanum dry extracts // J. Sep. Sci. 2007, N. 5, P. 708-712.
Urakova I.N., Pozharitskaya O.N., Shikov A.N., Kosman V.M., Makarov V.G. Development and validation of an LC method for simultaneous determination of ascorbic acid and three phenolic acids in sustained tablets at single wavelength // Chromatographia, 2008, Vol. 67, N. 9/10, P. 709-713.
Urakova I.N., Pozharitskaya O.N., Shikov A.N., Kosman V.M., Makarov V.G. Comparison of TLC and HPLC for separation and quantification of chlorogenic acid in green coffee bean extracts // Sep.Sci. 2008, Vol. 31, P. 231-241.
Косман В.М., Фаустова Н.М., Зенкевич И.Г., Макаров В.Г. Применение УФ-спектральных показателей окисленности в оценке доброкачественности жирных растительных масел // Масложировая пром-ть, 2009, № 1, С. 17-21.
Косман В.М., Фаустова Н.М., Пожарицкая О.Н., Шиков А.Н, Галамбози Б. Влияние ферментации на фитохимический состав сырья травы череды // Материалы IV всероссийской конференции «Новые достижения в химии и химической технологии растительного сырья». Барнаул, Алтайский Гос. Ун-т, 2009. С.258-260.
Косман В.М., Фаустова Н.М., Пожарицкая О.Н., Шиков А.Н., Галамбози Б., Макаров В.Г. Фитохимический анализ плодов облепихи крушиновидной российских сортов, культивируемых в Финляндии, и содержащих их пищевых продуктов // Вопросы питания, 2009, Т. 78, № 3, С.38-42.
Shikov A.N., Demchenko D., Ivanova S., Karlina M., Kosman V., Pozharitskaya O., Urakova I. HPTLC determination of ginkgolides A, B, C and bilobalide in Ginkgo biloba // CBS (Camag Bibliography Service), 2009, N 102, P. 10-12.
Shikov A.N., Kundacikova M., Palama T.L., Pozharitskaya O.N., Kosman V.M., Makarov V.G., Galambosi B., Kim H.J., Janf Y.P., Choi Y.H., Verpoorte R. Phenolic constituents of Gnaphalium uliginosum L. // Phytochemistry letters, 2010, Vol. 3, P. 45-47.
Pozharitskaya O.N., Shikov A.N., Makarova M.N., Kosman V.M., Faustova N.M., Tesakova S.V., Makarov V.G., Galambosi B. Anti-inflammatory activity of a HPLC-fingerprint aqueous infusion of aerial part of Bidens tripatrita L. // Phytomedicine, 2010, 17, Р. 463-468.
Косман В.М., Пожарицкая О.Н., Шиков А.Н., Фаустова Н.М., Иванова С.А., Демченко Д.В., Галамбози Б., Макаров В.Г. Изменчивость состава биологически активных веществ Bidens tripartita (Asteraceae) при интродукции на северо-западе России и в Финляндии // Растит. ресурсы, 2010, вып.1., С. 77-86.
Ioset K.N., Nyberg N.T., Van Diermen D., Malnoe P., Hostettmann K., Shikov A.N., Jaroszewski J.W. Metabolic profiling of Rhodiola rosea rhizomes by 1H NMR spectroscopy// Phytochemical analysis, 2011, 22 (2), 158-165.
Dorman H.J.D., Shikov A.N., Pozharitskaya O.N., Hiltunen R. Antioxidant and pro-oxidant evaluation of a Potentilla alba L. rhizome extract.// Chemistry & Biodiversity, 2011, 8(7), 1344-1356.
Shikov A.N., Ossipov V.I., Martiskainen O., Pozharitskaya N., Ivanova S.A., Makarov V.G. The offline combination of thin-layer chromatography and high-performance liquid chromatography with diode array detection and micrOTOF-Q mass spectrometry for the separation and identification of spinochromes from sea urchin (Strongylocentrotus droebachiensis) shells.// Journal of Chromatography A, 2011,1218(50), 9111-9114.
Косман В.М., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Изучение состава биологически активных веществ сухих экстрактов эхинацеи узколистной и шалфея лекарственного // Химия растит. Сырья, 2012, №1, 153-160.
Chernetsova E.S., Crawford E.A., Shikov A.N., Pozharitskaya O.N., Makarov V.G., Morlock G.E. ID-CUBE direct analysis in real time high-resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.// Rapid Communications in Mass Spectrometry, 2012, 26(11),1329-1337.
Косман В.М., Фаустова Н.М., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Накопление биологически активных веществ в подземных частях лапчатки белой (Potentilla alba L.) в зависимости от срока культивирования.// Химия растительного сырья.- 2013.- N2.- С. 139-146.
Pozharitskaya O.N., Ivanova S.A., Shikov A.N., Makarov V.G. Evaluation of Free Radical-Scavenging Activity of Sea Urchin Pigments Using HPTLC with Post-chromatographic Derivatization// Chromatographia.- 2013.- Vol.76 (19-20).- P.1353-1358.
Chernetsova, E.S., Shikov, A.N. Crawford, E.A., Grashorn, S., Laakso, I., Pozharitskaya, O.N., Makarov, V.G., Hiltunen, R., Galambosi, B., Morlock, G.E. Characterization of volatile and semi-volatile compounds in green and fermented leaves of Bergenia crassifolia L. by gas chromatography-mass spectrometry and ID-CUBE direct analysis in real time-high resolution mass spectrometry.// European Journal of Mass Spectrometry. 2014. – Vol. 20(2).- P. 199-205.
Shikov A.N., Pozharitskaya O.N., Makarov V.G., Wagner H., Verpoorte R., Heinrich M. Medicinal Plants of the Russian Pharmacopoeia; their history and applications. // Journal of Ethnopharmacology. – 2014, Vol.154 (3). – P.481-536.
Shikov A.N., Pozharitskaya O.N., Makarova M.N., Makarov V.G., Wagner H. Bergenia crassifolia (L.) Fritsch – Pharmacology and phytochemistry.// Phytomedicine. – 2014, Vol. 21(12). – P. 1534-1542.
Salminen J.-P., Shikov A.N., Karonen M., Pozharitskaya O.N., Kim J., Makarov V.G., Hiltunen R. Galambosi B. Rapid profiling of phenolic compounds of green and fermented Bergenia crassifolia L. leaves by UPLC-DAD-QqQMS and HPLC-DAD-ESI-QTOF-MS. // Natural Product Research.- 2014.- Vol. 28(19).- P.1530-1533.
Shikov A.N., Pozharitskaya O.N., Makarova M.N., Makarov V.G., Yang W-Z., Guo D-A. Oplopanax elatus (Nakai) Nakai: chemistry, traditional use and pharmacology// Chinese Journal of Natural Medicines.- 2014.- Vol. 12(10).- P. 721-729.
Косман В.М., Фаустова Н.М., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Разработка, валидация и применение методики иммуноферментного анализа для стандартизации биопрепарата // Разработка и регистрация лекарственных средств.- 2015.-№2(11).- С. 62-68.
Косман В.М., Пожарицкая О.Н., Шиков А.Н., Макаров В.Г. Cравнительное изучение содержания флавоноидов и кумаринов в некоторых препаратах ромашки аптечной // Химия растительного сырья. – 2015, № 1. – С. 107-112.
Pozharitskaya O.N., Shikov A.N., Laakso I., Seppänen-Laakso T., Makarenko I.E., Faustova N.M., Makarov V.G. Bioactivity and chemical characterization of gonads of green sea urchin Strongylocentrotus droebachiensis from Barents Sea //Journal of Functional Foods. – 2015, Vol. 17. – P. 227-234.
Krishtopina A.S., Urakova I.N., Kosman V.M., Katelnikova A.E., Pozharitskaya O.N., Shikov A.N. Isolation of peptide from Green sea urchins Strongilocentrotus Droebachiensis // Abstracts. Phitopharm. 2016. Obzory po klinicheskoj farmacologii i lekarstvennoj terapii. – 2016. –Vol. 14. – P. 30.ISOLATION OF PEPTIDE FROM GREEN SEA URCHINS STRONGYLOCENTROTUS DROEBACHIENSIS
Ushakova N.V., Faustova N.M., Mityushova E.V., Pozharitskaya O.N. Prostaglandins and hormones in extracts of sea urchins gonads, Strongilocentrotus Droebachiensis(O.F. Muller, 1776) // Abstracts. Phitopharm. 2016. Obzory po klinicheskoj farmacologii i lekarstvennoj terapii. – 2016. – Vol. 14. – P. 43-44.PROSTAGLANDINS AND HORMONES IN EXTRACTS OF SEA URCHINS GONADS STRONGYLOCENTROTUS DROEBACHIENSIS
Уракова И.Н., Крышень К.Л., Пожарицкая О.Н., Гаврилин М.В., Шиков А.Н., Макарова М.Н., Макаров В.Г. Сравнительное изучение взаимодействия с инсулиновым рецептором ряда отечественных субстанций инсулина человеческого и его аналогов методом поверхностного плазмонного резонанаса // Разработка и регистрация лекарственных средств. -2018, № 3(24). – С. 112-119.
Уракова И.Н., Крышень К.Л., Пожарицкая О.Н., Гаврилин М.В., Шиков А.Н., Макарова М.Н., Макаров В.Г. Сравнительное изучение взаимодействия с инсулиновым рецептором ряда отечественных субстанций инсулина человеческого и его аналогов методом поверхностного плазмонного резонанаса // Разработка и регистрация лекарственных средств. -2018, № 3(24). – С. 112-119.
Urakova I.N., Pozharitskaya O.N., Sukhov V.J., Makarova M.N., ShikovA.N., Makarov V.G. Distribution of the labeled [123i] peptide-protein drug cellex in rats // DRUG DEVELOPMENT & REGISTRATION. – 2018, Vol. 4(25). – P. 89-94. SUMMARY. The aim of this work was to study the distribution of the labeled 123I peptide-protein drug Cellex in organs and tissues of rats after single intravenous injection and accumulation. After a single intravenous injection of radiolabeled [123I]Cellex it was found that the drug is characterized by rapid distribution into tissues and organs. The highest content of the drug in all organs and tissues was observed in 15 minutes after its administration. It was established that the drug penetrates the blood-brain barrier and about 6% of the drug accumulates in the brain, which is 10% of the total amount of the drug found in all organs in 15 minutes after administration [Full text is available in Russian].
Kosman V.M., Karlina M.V., Pozharitskaya O.N. Effect of sample preparation and chromatographic conditions on background signal level in HPLC-UV analysis of blood plasma // Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo primeneniya = Тhе Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. – 2020, 10(2). – P.121-128. https://doi.org/10.30895/1991-2919-2020-10-2-121-128. ABSTRACT. The HPLC-UV method is widely used for quantitative analysis of drug substances found in biological samples obtained in pharmacokinetics studies, bioequivalence studies, or during therapeutic drug monitoring. The main limitation is associated with a significant background effect of biological matrixes, limiting the sensitivity of HPLC-UV methods. The aim of the study was to evaluate sample preparation and chromatographic conditions in terms of background signal level during HPLC-UV analysis of human and laboratory animal blood plasma. Material and methods: three types of blood plasma (rat, rabbit and human), three chromatographic modes, and three detection wavelengths were used to assess the effect of the precipitation agent, centrifuge conditions, and the inclusion of the solid-phase extraction (SPE) step into the sample preparation procedure on the background signal level during HPLC-UV analysis. Results: it was established that the background signal was practically unaffected by the chromatographic mode, centrifugation intensity, or introduction of the SPE step. The background signal levels for human and rabbit blood plasma tended to be lower than that for rat blood plasma. The factors that had the greatest effect on the background signal level were the choice of the precipitation agent during sample preparation, and the detection wavelength. It was shown that acetonitrile is preferable to methanol, and that the near UV region should be avoided. Conclusions: the identified key aspects of sample preparation procedures and chromatographic conditions can be used in the development and validation of bioanalytical methods for preclinical and clinical studies [Full text is available in Russian].
Elameen A, Kosman V.M., Thomsen M., Pozharitskaya O.N., Shikov A.N. Variability of Major Phenyletanes and Phenylpropanoids in 16-Year-Old Rhodiola rosea L. Clones in Norway // Molecules. – 2020, 25 (3463). – P. 1-12 doi:10.3390/molecules25153463
Kosman V.M., Karlina M.V., Makarova M.N. Experience in developing bioanalytical methods by HPLC with UV
detection. Farmatsiya. – 2020, 69 (3)– P. 23-35.
