Study of pharmaceuticals used in treating dermal diseases

Study of skin irritating properties of PA;
Study of skin sensitizing properties of PA;
Study of anti-inflammatory properties;
Study of the efficiency in the model of contact dermatitis:
- 2,4-dinitrochlorobenzene (DNCB)-induced model,
- fluorescein isothiocyanate (FITC)-induced model.
Assessment of wound healing action of PA in the model of linear skin wound;
Assessment of local anaphylactic reaction;
Model of passive skin anaphylaxis;
Acne Model
Study of specific activity in treating psoriasis:
- imikvamod-induced psoriasis model.

Publications by topic:

Krishen K.L., Azapkina A.A., Makarova M.N., Shikov A.N., Pozharitskaya O.N., Makarov V.G. Effect of different Melissa officinalis extract formulations on 2.4-dinitrofl uorobenzene induced allergic contact dermatitis in balb/c mice // Phytopharm. 2010. 14^thInternational congress. Book of Leiden. Netherlands. 1-3 July 2010. P. 65-66
Крышень К.Л., Дадали В.А., Бейшебаева Ч.Р., Павлова Р.Н., Смертина М.Н., Макарова М.Н., Карякина Л.А. Исследование взаимодействия диаллилдисульфида и омегалицина с ретиноидными рецепторами // Hi-Tech. Сборник статей под ред. А.П. Кудинова, Том 4, Санкт-Петербург, 2011 С.224-225.
Крышень К.Л., Демченко Д.В., Рыбакова А.В., Дадали В.А., Рыдловская А.В., Пожарицкая О.Н., Макарова М.Н., Шиков А.Н., Макаров В.Г. Оценка противоаллергенных свойств нового полипептидного препарата из печени тресковых // Экспериментальная и клиническая дерматокосметология. -2012, № 2. – С. 38-42.
Рыбакова А.В., Авдеева О.И., Макарова М.Н., Макаренко И.Е. Изучение безопасности противовоспалительного препарата Афлогилекс-гель 0,02% // Материалы IV съезда фармакологов России «Инновации в современной фармакологии». 18-21 сентября 2012 г., республика Татарстан, г. Казань. –М.: 2012. – С. 160-161.
Рыбакова А.В., Ковалева М.А., Макарова М.Н. Оценка ранозаживляющего действия геля Афлогилекс на модели линейной кожной раны // Сборник трудов III Международной Интернет-конференции «Современные проблемы анатомии, гистологии и эмбриологии животных. Казань, 3-6 апреля 2012 г., Казань, – 2012. – С. 97.
Рыдловская А.В., Авдеева О.И., Крышень К.Л., Ацапкина А.А., Макарова М.Н., Макаров В.Г. Изучение токсичности фракции лабдановых спиртов, выделенной из древесной зелени хвойных пород // Экспериментальная и клиническая дерматокосметология. -2011, №6.
Стефанов С.Ю., Крышень К.Л., Драй Р.В., Аликбаева Л.А., Макарова М.Н., Макаров В.Г. Субтканевые комплексы для оценки безопасности косметических средств // Экспериментальная и клиническая дерматокосметология №1, 2011 С.33-35.
Kovaleva M.A., Kryshen K.L., Makarova M.N., Alyakrinskaya A.A. A review of experimental models for the study of drugs used in skin diseases veterinary // International Bulletin of Veterinary Medicine. – 2016, № 3. – P. 160-167.
Kryshen K.L., Muzhikyan A.A., Alyakrinskaya A.A., Kovaleva M.A., Makarova M.N., Makarov V.G. Intracutaneous injection of Staphylococcus aureus bacteria into the scapophoccular angle of the auricle as a model of acne in laboratory animals // International Veterinary Journal. –2017, No. 1. – P. 84-91.
Zueva A.A., Kryshen K.L., Makarova M.N., Makarov V.G. Approbation of the iimiquimod-induced psoriasislike skin inflammation in BALB/с mice // Translyatsionnaya meditsina=Translational Medicine. – 2019, 6(4)– P. 50–58. ABSTRACT. Background. Antipsoriatic medicines that have been successfully tested by imiquimod-induced psoriasislike skin inflammation in BALB/c mice may be used for therapy of psoriasis induced by the immune response as inflammatory cascade into skin layers. Objective. Imiquimod-induced psoriasis-like skin inflammation approbation in BALB/c mice and search of more informative method of pathologic progress assessment for further extrapolating data to clinical cases. Design and methods. Psoriasis-like skin inflammation in mice was induced by topical applying of the Aldara® cream (5 % imiquimod) to back skin for 7 days. Psoriasis Area and Severity Index (PASI), histological study, calculation of relative spleen and thymus mass to the body weight, hematological analysis and skin disease area determination were used for registration of pathologic building. Results. During the study, was detected the increase of PASI score of animals with pathology to 18 with the formation of psoriasis-like plaques, significantly decrease body weight and relative thymus mass, significantly increase relative spleen mass, leukocytosis and leukocytic blood profile change, significant increase epidermal thickness, hyperkeratosis and inflammatory infiltration different degree. Conclusion. Results of approbation studied pathologic model with using of hematological analysis and skin disease area determination consistent with similar studies’ data and partly clinical sings in patient with psoriasis [Full text is available in Russian].
Zueva A.A., Katelnikova A.E., Gushchin Y.A., Makarova M.N. Approbation of hypertrophic scar model in the rabbit ear with development of a planimetric scale. Laboratory Animals for Science. – 2020,№4.https://doi.org/10.29296/2618723X-2020-04-01Abstract. In current study induction of ear hypertrophic scars in rabbit was carried out by local removal of deep layers of skin and perichondrium. The animals were monitored for 17 days. After 17th postwounding day animals were divided into 2 group (N=8 per group): (1) control group, (2) hormonal treatment group with Diprospan^® in dose 0,33 mg per animal. The histological study and measuring of scar thickness with scar elevation index (SEI) calculation were carried out for assessment of the pathology and therapeutic activity of tested medicine on the 27th and 37th postwounding day. Scoring system was developed and adapted for animals, in which the following were considered: elevation, surface, contour, texture and color of the wounds. In results there were observed that the skin changes in control group conform to literature: scars were erythemic, which became poorly later, thick and contoured. Planimetric summary score was 6.5-7.0, and it decreased to 4.5 at the end. SEI increased also large number of fibroblast and irregularly arranged collagen fibers, moderate inflammation infiltration were registered in the superficial layer of scar tissue throughout the study. Use the Diprospan^® in the treatment of hypertrophic scars indicates a complete cosmetic correction in rabbit scar healing. There were observed significantly increased planimetric summary score to 0, significantly increased SEI, lower number of fibroblast and regularly arranged collagen fibers and absence inflammation. It is comparable with the clinical effect of complex scar therapy in patients animals[Full text is available in Russian].

Krishen K.L., Azapkina A.A., Makarova M.N., Shikov A.N., Pozharitskaya O.N., Makarov V.G. Effect of different Melissa officinalis extract formulations on 2.4-dinitrofl uorobenzene induced allergic contact dermatitis in balb/c mice // Phytopharm. 2010. 14^thInternational congress. Book of Leiden. Netherlands. 1-3 July 2010. P. 65-66
Крышень К.Л., Дадали В.А., Бейшебаева Ч.Р., Павлова Р.Н., Смертина М.Н., Макарова М.Н., Карякина Л.А. Исследование взаимодействия диаллилдисульфида и омегалицина с ретиноидными рецепторами // Hi-Tech. Сборник статей под ред. А.П. Кудинова, Том 4, Санкт-Петербург, 2011 С.224-225.
Крышень К.Л., Демченко Д.В., Рыбакова А.В., Дадали В.А., Рыдловская А.В., Пожарицкая О.Н., Макарова М.Н., Шиков А.Н., Макаров В.Г. Оценка противоаллергенных свойств нового полипептидного препарата из печени тресковых // Экспериментальная и клиническая дерматокосметология. -2012, № 2. – С. 38-42.
Рыбакова А.В., Авдеева О.И., Макарова М.Н., Макаренко И.Е. Изучение безопасности противовоспалительного препарата Афлогилекс-гель 0,02% // Материалы IV съезда фармакологов России «Инновации в современной фармакологии». 18-21 сентября 2012 г., республика Татарстан, г. Казань. –М.: 2012. – С. 160-161.
Рыбакова А.В., Ковалева М.А., Макарова М.Н. Оценка ранозаживляющего действия геля Афлогилекс на модели линейной кожной раны // Сборник трудов III Международной Интернет-конференции «Современные проблемы анатомии, гистологии и эмбриологии животных. Казань, 3-6 апреля 2012 г., Казань, – 2012. – С. 97.
Рыдловская А.В., Авдеева О.И., Крышень К.Л., Ацапкина А.А., Макарова М.Н., Макаров В.Г. Изучение токсичности фракции лабдановых спиртов, выделенной из древесной зелени хвойных пород // Экспериментальная и клиническая дерматокосметология. -2011, №6.
Стефанов С.Ю., Крышень К.Л., Драй Р.В., Аликбаева Л.А., Макарова М.Н., Макаров В.Г. Субтканевые комплексы для оценки безопасности косметических средств // Экспериментальная и клиническая дерматокосметология №1, 2011 С.33-35.
Kovaleva M.A., Kryshen K.L., Makarova M.N., Alyakrinskaya A.A. A review of experimental models for the study of drugs used in skin diseases veterinary // International Bulletin of Veterinary Medicine. – 2016, № 3. – P. 160-167.
Kryshen K.L., Muzhikyan A.A., Alyakrinskaya A.A., Kovaleva M.A., Makarova M.N., Makarov V.G. Intracutaneous injection of Staphylococcus aureus bacteria into the scapophoccular angle of the auricle as a model of acne in laboratory animals // International Veterinary Journal. –2017, No. 1. – P. 84-91.
Zueva A.A., Kryshen K.L., Makarova M.N., Makarov V.G. Approbation of the iimiquimod-induced psoriasislike skin inflammation in BALB/с mice // Translyatsionnaya meditsina=Translational Medicine. – 2019, 6(4)– P. 50–58. ABSTRACT. Background. Antipsoriatic medicines that have been successfully tested by imiquimod-induced psoriasislike skin inflammation in BALB/c mice may be used for therapy of psoriasis induced by the immune response as inflammatory cascade into skin layers. Objective. Imiquimod-induced psoriasis-like skin inflammation approbation in BALB/c mice and search of more informative method of pathologic progress assessment for further extrapolating data to clinical cases. Design and methods. Psoriasis-like skin inflammation in mice was induced by topical applying of the Aldara® cream (5 % imiquimod) to back skin for 7 days. Psoriasis Area and Severity Index (PASI), histological study, calculation of relative spleen and thymus mass to the body weight, hematological analysis and skin disease area determination were used for registration of pathologic building. Results. During the study, was detected the increase of PASI score of animals with pathology to 18 with the formation of psoriasis-like plaques, significantly decrease body weight and relative thymus mass, significantly increase relative spleen mass, leukocytosis and leukocytic blood profile change, significant increase epidermal thickness, hyperkeratosis and inflammatory infiltration different degree. Conclusion. Results of approbation studied pathologic model with using of hematological analysis and skin disease area determination consistent with similar studies’ data and partly clinical sings in patient with psoriasis [Full text is available in Russian].
Zueva A.A., Katelnikova A.E., Gushchin Y.A., Makarova M.N. Approbation of hypertrophic scar model in the rabbit ear with development of a planimetric scale. Laboratory Animals for Science. – 2020,№4.https://doi.org/10.29296/2618723X-2020-04-01Abstract. In current study induction of ear hypertrophic scars in rabbit was carried out by local removal of deep layers of skin and perichondrium. The animals were monitored for 17 days. After 17th postwounding day animals were divided into 2 group (N=8 per group): (1) control group, (2) hormonal treatment group with Diprospan^® in dose 0,33 mg per animal. The histological study and measuring of scar thickness with scar elevation index (SEI) calculation were carried out for assessment of the pathology and therapeutic activity of tested medicine on the 27th and 37th postwounding day. Scoring system was developed and adapted for animals, in which the following were considered: elevation, surface, contour, texture and color of the wounds. In results there were observed that the skin changes in control group conform to literature: scars were erythemic, which became poorly later, thick and contoured. Planimetric summary score was 6.5-7.0, and it decreased to 4.5 at the end. SEI increased also large number of fibroblast and irregularly arranged collagen fibers, moderate inflammation infiltration were registered in the superficial layer of scar tissue throughout the study. Use the Diprospan^® in the treatment of hypertrophic scars indicates a complete cosmetic correction in rabbit scar healing. There were observed significantly increased planimetric summary score to 0, significantly increased SEI, lower number of fibroblast and regularly arranged collagen fibers and absence inflammation. It is comparable with the clinical effect of complex scar therapy in patients animals[Full text is available in Russian].