- Acute toxicity is examined according to the Manual for conduting preclinical studies of drugs. Under the editorship of Mironov A.N., Bunatyan N.D. et al., M., publishing house “Grif and K”, 2012;
- Toxicological study of pharmaceuticals to identify threshold doses (DRF-protocol) FDA Guideline for Industry Toxicokinetics: The Assessment of Systemic Exposure in Toxicity Studies and ICH – M4S;
- Study of acute toxicity is performed according to OECD protocols on testing of chemicals:
| 402 | Acute dermal toxicity |
| 403 | Acute inhalation toxicity |
| 404 | Acute dermal irritation|corrosion |
| 405 | Acute eye irritation|corrosion |
| 420 | Acute oral toxicity – fixed dose procedure |
| 423 | Acute oral toxicity – class method |
| 425 | Acute oral toxicity, up and down procedure |
| 436 | Acute inhalation toxicity, class method |
| 439 | In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method |