- study of specific pharmacological activity of antiplatelet agents and anticoagulants in vitro and in vivo;
- study of specific pharmacological activity of PA in the model of experimental thrombosis;
- monitoring of treatment using direct anticoagulants by evaluation of anti-Xa and anti-IIa activity of test substances.
Publications by topic:
- Кашкин В.А., Соколова А.В., Абрашова Т.В., Макарова М.Н., Макаров В.Г. Особенности гемостаза у крыс// Международный вестник ветеринарии. -2013, №4. –С. 88-93.
- Справочник Физиологические, биохимические и биометрические показатели нормы экспериментальных животных. Под ред.: д.м.н. Макарова В.Г., д.м.н. Макаровой М.Н. Абрашова Т.В., Гущин Я.А., Ковалева М.А., Рыбакова А.В., Селезнева А.И., Соколова А.П., Ходько С.В. СПб.: Из-во «ЛЕМА», 2013. -116 с.
- Obluchinskaya E.D., Маkarova M.N., Pozharitskaya O.N., Shikov A.N. Effects of ultrasound treatment on the chemical composition and anticoagulant properties of dry Fucus extract // Pharmaceutical Chemistry Jornal. -2015. –Vol. 49, № 3. –P. 183-186.
- Облучинская Е.Д., Макарова М.Н., Пожарицкая О.Н., Шиков А.Н. Влияние ультразвуковой обработки на химический состав и антикоагулянтные свойства сухого экстракта фикуса // Химико-фармацевтический журнал. – 2015. – Т. 49, № 3. – С. 61-64.
- Макаренко И.Е., Калатанова А.В., Ванатиев Г.В., Мужикян А.А., Шекунова Е.В., Буренков П.В., Макарова М.Н., Макаров В.Г. Выбор оптимального вида животных для моделирования экспериментального артериального тромбоза// Международный вестник ветеринарии. – 2016, № 2. – С. 116-125.
- Burenkov P.V., Shekunova E.V., Faustova N.M., Muzhikyan A.A., Makarov V.G. Antiplatelet effect of acid glycoprotein in rabbit // Phitopharm. 2016. Obzory po klinicheskoj farmacologii i lekarstvennoj terapii. – 2016, Vol. 14. – P. 14. ANTIPATELET EFFECT OF ACID GLYCOPROTEIN IN RABBITS
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Kashkin VA, Shekunova EV, Muzhikyan AA, Makarova MN, Makarov VG. Simulation of ferric chloride induced arterial thrombosis in rabbits // Veterinary Pathology. -2016, № 3 (57). -P. 53-60.
- Burenkov VV, Shekunova EV, Lyutov AG, Mouzhikyan AA, Makarova MN, Makarov V.G. Evaluation of pharmacological effects of Orozin on the model of arterial thrombosis // Thrombosis, hemostasis and rheology. – 2017, No. 2 (70). – P. 31-38.
- Макарова М.Н., Макаров В.Г. Диет-индуцированные модели метаболических нарушений. Сообщение 6: экспериментальная анемия. Лабораторные животные для научных исследований. – 2019, №2. https://doi.org/10.29296/2618723X-2019-02-04
- Makarova M., Makarov V. Diet-induced Models of Metabolic Disorders. Report 6: Experimental Anemia. Laboratory Animals for Science. – 2019, 2. https://doi.org/10.29296/2618723X-2019-02-04 ABSTRACT. The widespread prevalence of anemia, mainly iron deficiency, among the world’s population determines the need for constant search for means of prevention and treatment of this disease, which is impossible without adequate experimental models. For modeling anemia use mostly rats and mice (Wistar, Sprague-Dawley, C57BL/6 and others.), rarely Guinea pigs, primarily females and cubs. Semi-synthetic diets (AIN-93G, TD.80396 and others) with reduced iron content (5.2–7.8–8 mg / kg diet), the standard diet of control animals contains 35 mg/kg of iron for rats and mice, about 100 mg/kg diet – for Guinea pigs. The article presents the characteristics and composition of semi-synthetic diets for modeling iron deficiency anemia in laboratory animals, which is manifested by growth retardation, weight loss, tolerance to physical activity and cognitive activity, splenomegaly and cardiomegaly, weakening of immunity. The data on the main morphological (the number of red blood cells, reticulocytes, platelets and their indices) and biochemical (the concentration of hemoglobin of blood and red blood cells, serum iron, ferritin, transferrin, the activity of redox enzymes) blood parameters to assess the effectiveness of the studied drugs. The parameters of changes in the state of blood and metabolism given in the article give an idea of the direction of these changes in the development of experimental anemia. For example, a significant decrease in body weight, a drop in hemoglobin levels, a tendency to reduce the average hemoglobin content in erythrocytes, their average volume, as well as iron content in serum and liver, serum ferritin concentration, against the background of a sharp increase in the total iron binding capacity of blood serum, reducing the activity of superoxide dismutase and glutathione peroxidase. These disorders, indicating the development of microcytic, hypochromic iron deficiency anemia in animals, allow to evaluate their dynamics in the subsequent testing of the studied therapeutic agents. [Full text is available in Russian].